Abstract
By means of a suitable zymographic technique we revealed a cytochrome c degrading system in rat liver mitochondria. After separation of the mitochondrial proteins by polyacrylamide gel electrophoresis and incubation of the gels in the presence of cytochrome c , three bands of protease activity were detected. Distinct zymogram patterns were obtained in parallel assays using lysosomes or cytosol instead of mitochondria. The soluble and the membrane components of mitochondria were analyzed by the same method to assess the intra-mitochondrial localization of these proteases.
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Schedule a callMore From: Biochemical and biophysical research communications
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