Abstract
SUMMARYSpecific staining methods (Feulgen, methyl green-pyronin, toluidine blue molybdate, acridine orange), digestion tests (DNase and RNase) and autoradiographic techniques (incorporation of tritiated thymidine, uridine, lysine, tryptophan; DNA detection by means of tritiated actinomycin D) have been used to study the embryo suspensor of Phaseolus coccineus. Most of material studied consisted of developing seeds in which the cotyledons of the embryo occupied one third to one half of the endospermatic cavity. In the typically club-shaped suspensor, both the « handle » portion (which contains cells with low to medium degree of endopolyploidy) and the « knob » portion (which contains polytene chromosome cells) have been analyzed.Indications have been found that the cells with low and medium degree of endopolyploidy undergo extra DNA synthesis (gene amplification) on many heterochromatic chromosome regions during early stages of embryogenesis. Later on, the extra DNA synthesized seems to be released, from t...
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