Cyto-toxic Effect of Nano Ceria bio fabricated with DNA

Abstract

Cancer is a condition arising from the deregulation of several genes which causes change in normal cells to cancerous ones. The objective of all cancer treatment research is to eradicate cancer with little effect on normal cells. Nanostructured ceria of very small dimension (≈5nm) synthesized by co-precipitation method using deoxyribo nucleic acid (DNA) as capping agent has shown a promising antioxidant activity over almost all free radicals. Excessive reactive oxygen species have significant influence in the origination and development of numerous ailments, including cancer. So, the cyto-toxic effect of synthesized nanoceria on normal cells as well as on colon cancer cells is investigated. Inverted phase contrast microscope succeeded by MTT assay method were used to observe the survivability of cells. The investigation reveals a significant decline in the viability of cells when nanoceria, in the concentration range of 6.25–100μg/ml, was used on human cancer cells for 24,48 and 72 hours. The results showed that the activity of nanostructured ceria significantly depends on dose and time. For normal cells the reduction is less i.e., more than 75% of the cells are viable even if the concentration increases to 100μg/ml. But for cancer cells the percentage of viability decreases. Our study come up with a result that DNA assisted synthesized nanoceria has considerable cytotoxic effect, which seems likely to be effective as an anticancer agent while protecting healthy tissues.