Abstract
Objective Growing evidence shows that enhancer RNAs (eRNAs) are pivotal for tumor progression. In this research, our team aimed to identify the survival-related eRNAs and further explore their potential function in glioblastoma (GBM). Methods RNA-sequencing data in 31 tumor types were acquired from TCGA datasets. The survival-related eRNAs were identified by the use of Kaplan-Meier survival analyses and Spearman's correlation analyses. KEGG pathway enrichment analysis was completed to investigate the underlying signal paths of the critical eRNA. Pancancer assays were applied to explore the association between CYP1B1-AS1 and CYP1B1. Results We identified 74 survival-related eRNAs and focused on CYP1B1-AS1 which displayed the greatest cor value. CYP1B1 was identified as a regulatory target of CYP1B1-AS1. KEGG analyses suggested that CYP1B1-AS1 might play an essential role through CK-CKR mutual effect, complement and coagulation cascades, TNF signal path, and JAK-STAT signal path. The pancancer verification outcomes revealed that CYP1B1-AS1 was related to survival in 4 cancers, i.e., LIHC, KIRP, KICH, and KIRC. Association was discovered between CYP1B1-AS1 and the targeted gene, CYP1B1, in 29 cancer types. Conclusion The outcomes herein provided the first evidence that overexpression of CYP1B1-AS1 might be a potential molecular biomarker for predicting the prognosis of patients with GBM.
Highlights
Glioblastoma (GBM) is the most common and malignant tumor of the human central nervous system, accounting for 81% of brain tumors [1]
Thanks to the development of the latest sequencing techniques, ncRNAs have remarkably aroused our interest in the academic world owing to the fact that they are capable of regulating genetic expression [5]. enhancer RNAs (eRNAs) pertains to ncRNA transcribed from the enhancer and serves as a specific type of lncRNA generated by transcript of enhancer elements [6]
39 eRNAs reached the standard (Spearman’s rank relational coefficient r > 0:40, p < 0:001; Table S2), and CYP1B1-AS1 displayed the greatest cor result and was deemed as the most correlated eRNA related to the targeted genes
Summary
Glioblastoma (GBM) is the most common and malignant tumor of the human central nervous system, accounting for 81% of brain tumors [1]. It is extremely aggressive, and the disease develops very fast [2]. Despite the fact that early diagnostic and therapeutic approaches of GBM have developed remarkably recently, the mean survival posterior to diagnoses is 12-15 months, with less than 5% sufferers living >5 years, primarily owing to the commonly seen late period illness with lymphatic or distant metastatic activities [3, 4]. Researches have revealed that they are vital for the mediation of targeted genetic stimulation and transcript [7, 8].
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