Abstract
Transformed root cultures of Astragalus mongholicus are induced by inoculation of sterile grown seedlings with Agrobacterium rhizogenes strains LBA 9402, ATCC 15834, R 1601 and TR 105. These strains show different abilities to induce hairy roots on this plant. They influence the growth rate, the saponin production, and the ratio of astragalosides. Root growth and saponin production are investigated under various culture conditions. Transformed roots grow better in MS medium without ammonium nitrate and without phytohormone. The saponin production is remarkably increased when sitosterol is added in the culture media. © 1997 Published by Elsevier Science Ltd. All rights reserved
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