Cyclicity, estrus expression and pregnancy rates in beef heifers with different reproductive tract scores following progesterone supplementation

Abstract

Two experiments were conducted to determine effects of progesterone (P4) on cyclicity, estrus expression rate (EER) and artificial insemination pregnancy rate (AIPR) in beef heifers with various reproductive tract scores (RTS; 1 to 5; 1, immature, acyclic; 5, mature, cyclic). In Experiment 1, Angus-cross heifers (n = 100, 20 per RTS category; mean (±SEM) age, 15 ± 0.8 mo) were randomly assigned to receive a CIDR (Days −17 to −10) or no CIDR (untreated control), with weekly blood samples and ultrasonography (Days 0–85). Among heifers with RTS 2 to 4, median interval to cyclicity were shorter (P < 0.05) for heifers in CIDR versus control. In Experiment 2, Angus-cross heifers (n = 11,098) were assigned RTS, body condition score (BCS; 1 to 9; 1, emaciated; 9, obese) and temperament score (calm versus excitable). Heifers with RTS 2–5 (n = 10,569) were allocated to CO-Synch (n = 5099) or CO-Synch + CIDR (n = 5470). Estrus was detected until AI (72 h after PGF2α), with pregnancy diagnosis ∼70 d later. Controlling for RTS (P < 0.0001), BCS (P < 0.0001), temperament (P < 0.0001), age (P < 0.0001), treatment by RTS (P < 0.01), treatment by BCS (P < 0.01), and treatment by age, EER differed between CO-Synch and CO-Synch + CIDR (71.0 vs 75.9%, respectively, P < 0.0001). Accounting for RTS (P < 0.0001), BCS (P < 0.0001), temperament (P < 0.0001), age (P < 0.0001), heifers detected in estrus (P < 0.0001), RTS by treatment (P < 0.01), BCS by treatment (P < 0.01), and age by treatment, AIPR differed between CO-Synch versus CO-Synch + CIDR (55.3 vs 61.0%, P < 0.0001). In conclusion, exogenous P4 hastened cyclicity in pre- and peri-pubertal beef heifers. Further, it increased EER and AIPR. However, RTS, BCS and age influenced EER and AIPR. Among RTS 4 and 5, EER was greater for CO-Synch + CIDR vs CO-Synch. Among RTS 3 to 5, AIPR was greater for CO-Synch + CIDR versus CO-Synch. Progesterone status or supplementation at onset of synchronization protocols was critical to pregnancy outcomes, emphasizing heifer development for early puberty or progesterone supplementation.