CXC chemokine receptor 5 expression in gastric cancer and its clinical significance

Abstract

Objective To investigate the expression of CXC chemokine receptor 5(CXCR5)in human gastric cancer cell lines and gastric carcinoma tissues and its clinical significance. Methods Flow cytometry was performed to analyze the CXCR5 expression in human gastric cancer cell lines, BGC-823, SGC-7901 and HGC-27.Immunohistochemistry and tissue-microarray were used to detect the CXCR5 expression in human gastric cancer tissues and adjacent normal tissues from 90 patients. Results The flow cytometry revealed that the CXCR5 expression was detectable in BGC-823, SGC-7901 and HGC-27 cells.The results of tissue microarray and immunohistochemistry showed that the ratio of higher CXCR5 expression in gastric cancer tissues was 52. 87%.The CXCR5 expression level in patients with tumor size≤2. 8 cm group was significantly lower than that in patients with tumor size> 2. 8 cm(χ2= 3. 995,P 0. 05).Survival analysis showed that the overall survival rate of the patients with high CXCR5 expression was lower than that of the patients with low CXCR5 expression[hazard ratio(HR)= 1. 810,95% confidence interval(CI):0. 980 3-3. 341 0,P> 0. 05]. Conclusion CXCR5 is involved in the oncogenesis and progression of human gastric cancer. Key words: Gastric cancer; CXC chemokine receptor 5