The mechanism of stomatin-like protein 2 gene regulating Wnt/β-catenin signaling pathway on proliferation and apoptosis of gastric cancer cells

Abstract

Objective To investigate the effect of stomatin-like protein 2 (SLP-2) gene on the proliferation and apoptosis of gastric cancer cells by Wnt/β-catenin signaling pathway. Methods The expression of SLP-2 gene in human gastric cancer tissues was detected by Western blotting; NC-siRNA, SLP-2-siRNA1 and SLP-2-siRNA2 were transfected into human gastric cancer SGC-7901 cells, and not transfected with any siRNA as the control group; the expression of SLP-2 protein after transfected cells was detected by Western blotting; cells transfected for 48 h, cell apoptosis was detected by flow cytometry; Cleaved Caspase-3, β-catenin, c-Myc, Cyclin D1protein expression was detected by Western blotting. Results The expression of SLP-2 protein in gastric carcinoma (0.283±0.016)was significantly higher than paracancerous tissues (0.082±0.016), (t=7.789, P=0.003); the inhibitory effect of SLP-2-siRNA2 group was more obvious, and was choiced as a follow-up study; the apoptosis rate (16.62±1.32)% of cells and Cleaved Caspase-3 (1.421±0.210) protein expression in SLP-2-siRNA group were significantly higher than the control group (1.56±0.56)%, (0.510±0.083), β-catenin (0.157±0.024), (0.039±0.016), Cyclin D1 (0.052±0.012) protein expression was significantly lower than the control group (0.311±0.036, 0.137±0.026, 0.183±0.021) (tapoptosis rate=9.324, Papoptosis rate=0.001; tCleaved Caspase-3=6.112, PCleaved Caspase-3=0.006; tβ-catenin=8.868, Pβ-catenin=0.003; tc-Myc=9.112, Pc-Myc=0.002; tCyclin D1=9.987, PCyclin D1=0.001). Conclusion SLP-2 is highly expressed in gastric cancer, Silencing SLP-2 expression can inhibit the proliferation and apoptosis of human gastric cancer cell line SGC-7901, and its mechanism is related to the regulation of Wnt/β-catenin signaling pathway. Key words: Stomatin-like protein 2; Wnt/β-catenin signaling pathway; Gastric cancer; Proliferation; Apoptosis