Abstract
Objective The objective of this study is to explore the rooting mechanism of Catalpa bungei, as well as the morphological characteristics and physiological and biochemical characteristics of cuttings during rooting process. Method The cuttings of C. bungei were treated with dissolved GGR-6 to promote rooting, and the enzyme activities, nutrients and rooting inhibitors in different rooting stages were measured. Result GGR-6 treatment significantly promoted the rooting of cuttings, and the rooting rate reached 82.04%, higher than that of the control treatment (P＜0.01). During the rooting process, the activities of superoxide dismutase (SOD), peroxidase (POD) and polyphenol oxidase (PPO) increased during the formation of a large number of callus, and the maximum values of SOD and PPO were 623.33×16.67 and 57.44×16.67 nkat·g−1, respectively. POD activity decreased during the formation of adventitious roots, and the three enzymes were positively correlated with the rooting rate. The soluble sugar and C/N ratio in cuttings decreased sharply at the callus stage, only about 40% of the initial value, and reached the maximum at 20 days of cutting. The soluble protein and total nitrogen showed a decreasing trend, and reached the minimum at 25−30 days. Under GGR-6 treatment, the change range of nutrients in cuttings was large, and the maximum values were higher than those of the control. Total nitrogen content was negatively correlated with rooting rate (P＜0.05), while soluble sugar, soluble protein and C/N were significantly positively correlated with rooting rate (P＜0.05). GC-MS analysis identified four rooting related substances, three of which might inhibit rooting and one might promote rooting. Conclusion The increase of antioxidant enzyme activities of cuttings can promote the occurrence of adventitious roots of C. bungei. Soluble sugar and soluble protein are the main nutrients of cuttings. Quercetin, linoleic acid and palmitic acid may inhibit the rooting of cuttings and phytosterol may promote the rooting of cuttings. [Ch, 2 fig. 4 tab. 32 ref.]
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