Abstract
目的 揭示菊科Asteraceae欧洲千里光Senecio vulgaris CYC2类RAY1基因过表达使舌状花发生不同程度变宽现象的机制，进一步探究其产生原因。 方法 克隆欧洲千里光SvRAY1基因，利用生物信息学、qRT-PCR、超表达载体构建、扫描电镜观察、转基因植株形态学观察与统计等方法与技术，进一步进行SvRAY1基因功能分析。 结果 qRT-PCR反应显示：SvRAY1基因主要在欧洲千里光舌状花及筒状花中表达，且生殖发育第S3和S4阶段舌状花中表达量最高；形态学观察表明转基因欧洲千里光SvRAY1超表达植株的舌状花比野生型长度较短、显著变宽。扫描电镜观察舌状花腹侧表皮细胞大小与形状，宽度显著变宽的株系中显示远轴端细胞排列紧密且细胞分裂旺盛，中轴端细胞形状由边缘弯曲变为平滑，细胞长度变短且分裂旺盛。 结论 欧洲千里光舌状花发育过程中，SvRAY1基因可能促进细胞横向分裂，进而舌状花细胞形态和排列发生不同程度的变化引起舌状花变宽。图6表2参28
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