Abstract
Foreword Preface Contributors List Conversion Factors Flow Chart: Application of Techniques Emily Wiley, Claremont Colleges Chapter 1 Volume/Weight Measurement Unit 1.1 Volume Measurement Andrew Zanella and Tom Davis, Claremont Colleges Unit 1.2 Weight Measurement Michael Guzy, Ohaus, Corp. Chapter 2 Concentration Measurement Unit 2.1 Spectrophotometry Rob Morris, Ocean Optics, Inc. Unit 2.2 Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy Sean R. Gallagher, UVP, LLC Chapter 3 Reagent Preparation Unit 3.1 Theoretical Discussion Deb Chakravarti, Keck Graduate Institute Unit 3.2 Practical Discussion Deb Chakravarti, Keck Graduate Institute Unit 3.3 Recipes In-House Chapter 4 Cell Culture Techniques Unit 4.1 Aseptic Technique Thomas Bykowski and Brian Stevenson, University of Kentucky College of Medicine Unit 4.2 Culture of Escherichia coli and Related Bacteria Sean P. Riley, Michael E. Woodman, and Brian Stevenson, University of Kentucky College of Medicine Chapter 5 Sample Preparation Unit 5.1 Centrifugation and Cell Fractionation In-House Unit 5.2 Purification and Concentration of Nucleic Acids Dennis H. Dowhan, University of Queensland Chapter 6 Chromatography Unit 6.1 Overview of Chromatography Buddhadeb Mallik, Bulbul Chakravarti, and Deb N. Chakravarti, Keck Graduate Institute of Applied Life Sciences Unit 6.2 Column Chromatography Deb Chakravarti, Keck Graduate Institute Chapter 7 Electrophoresis Unit 7.1 Overview of Electrophoresis Sean R. Gallagherr, UVP, LLC Unit 7.2 Agarose Gel Electrophoresis Jennifer A. Armstrong, The Claremont Colleges, and Joseph R. Schulz, Occidental College Unit 7.3 SDS-PAGE Sean Gallagher, UVP, LLC Unit 7.4 Staining of Gels Sean Gallagher, UVP, LLC Chapter 8 Blotting Unit 8.1 Overview of Blotting Maria Cristina Negritto, Pomona College, and Glenn M. Manthey, City of Hope, Beckman Research Institute Unit 8.2 Nucleic Acid Blotting: Southern and Northern Laura L. Mays Hoopes, Pomona College Unit 8.3 Protein Blotting: Immunoblotting Sean Gallagher, UVP, LLC Unit 8.4 Labeling DNA and Probe Preparation Karl A. Haushalter, Harvey Mudd College Chapter 9 Microscopy Unit 9.1 Conventional Light Microscopy Eric S. Cole, St. Olaf College Unit 9.2 Immunofluorescence Microscopy David J. Asai, Harvey Mudd College Chapter 10 Enzymatic Reactions Unit 10.1 Working with Enzymes David Skrincosky and Cindy Santangelo, Worthington Biochemical Corp. Unit 10.2 Overview of PCR Christine D. Kuslich, Molecular Profiling Institute, Buena Chui, GE Healthcare, and Carl T. Yamashiro, Molecular Profiling Institute Unit 10.3 Real-Time PCR Dean Fraga, College of Wooster, Tea Meulia, Ohio Agricultural Research and Development Center, Steven Fenster, Ashland University Chapter 11 Bioinformatics Unit 11.1 Using NCBI Blast Nicholas A. Stover, Bradley University, and Andre R.O. Cavalcanti, Pomona College
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