Abstract
Objective To explore the effects and mechanism of curcumin on the proliferation and paracrine of adipose-derived stem cells (ADSCs). Methods ADSCs were isolated through enzymes from SD rats inguina. The phenotypes of ADSCs were analyzed by flow cytometry. The effects of different concentrations of curcumin on cell growth curve were determined through MTT. Following by pretreatment of curcumin on ADSCs for 24 h, the SnPP, an inhibitor of heme oxygenase-1 (HO-1), was added to suppress HO-1 activity. PCR and western blotting was used to assess the changes of HO-1 expression during the curcumin treatment. The effects of HO-1 expression on the curcumin promoting proliferation by MTT. qPCR and Elisa were applied to investigate the influences of curcumin on the secretion of VEGF and HGF. Results After three passages, most of adherent ADSCs expressed CD29 and CD90. But they were negative for CD31, CD34 and CD45. The promoting proliferation of curcumin on ADSCs depended on time. The optimal treatment concentration of curcumin was 10 μmol/L. After added SnPP, the increased proliferation of ADSCs by curcumn pretreatment decreased significantly (P=0.001). And the enhancing VEGF and HGF secreted by curcumin pretreated ADSCs (P=0.001 and P=0.007, respectively) were reduced by treatment of SnPP [Cur-ADSCs vs. Cur-ADSCs+SnPP: VEGF: (712.2±43.1) ng/L vs. (442.3±33.2) ng/L, P=0.001; HGF: (58.3±3.7) ng/L vs. (33.7±3.1) ng/L, P=0.001]. Importantly, the change trend of HO-1 was parallel with the promoting proliferation and paracrine of ADSCs by pretreatment of curcumin. Conclusions Curcumin promotes the proliferation and paracrine of ADSCs partly via HO-1 signaling pathway. Curcumin is a promising candidate drug for enhancing the therapeutic effects of stem cells. Key words: Curcumin; Heme oxygenase-1; Adipose-derived stem cells; Cell proliferation; Paracrine
Published Version
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