Abstract
Objective To explore the imaging effectiveness of indocyanine green (ICG) in pancreatic cancer cells and curative effect evaluation of neoadjuvant chemotherapy in pancreatic cancer. Methods The basic characteristics of ICG was characterized and cell counting kit-8 (CCK-8) assay was used to observe the toxic effects of ICG and gemcitabine (GEM) in pancreatic cancer cell lines (ASPC-1 and PANC-1). After incubated with ICG for 4 hours, the ICG uptake in this two cells was observed by a high sensitivity optical imaging system (IVIS) respectively. A subcutaneous xenograft model of pancreatic cancer was established and then divided into phosphate buffer (PBS) + ICG injection group (control group) and GEM + ICG injection group (treatment group). After injection of PBS (100 mg/kg) or GEM (100 mg/kg) 24 hours, ICG (7 μg/ml) 200 μl was injected into mice by tail vein, then the fluorescence intensity in tumor tissue was observed continually by IVIS. Results ICG almost non-toxic to cells can be excited by near-infrared light also has a good dispersion and light stability. The fluorescence intensity in both control and GEM group of ASPC-1 cells was (2.437±0.039)×107 and (0.564±0.327)×107 respectively (F=13.262, P<0.05). Similarly, The fluorescence intensity in both control and GEM group of PANC-1 cells was (2.831±0.029)×107 and (0.376±0.019)×107 respectively (F=5.381, P<0.05). Fluorescence intensity of tumor was continuously measured by IVIS at different time points. After 4, 5 and 6 hours, the tumor fluorescence intensity in GEM group was stronger than that in control group respectively (P<0.05). Conclusion ICG can be absorbed by pancreatic cancer cells and accumulate in pancreatic tumor tissues after treated with GEM. Therefore, ICG fluorescence imaging can be used to evaluate efficacy of neoadjuvant chemotherapy in Pancreatic Cancer. Key words: Pancreatic cancer; Indocyanine green; Gemcitabine; Neoadjuvant chemotherapy; Fluorescence imaging
Published Version
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