Cumulation of Tabernanthe Iboga alkaloid and its metabolite in organs of mice

Abstract

The accumulation of Tabernanthe Iboga alkaloid ibogaine and its metabolite noribogaine in liver, kidney, spleen, heart, brain and muscle after administration directly into the stomach in white laboratory mice were studied. For this purpose a high-performance liquid chromatography (HPLC) method with fluorescein as internal standart were chosen. Solid phase extraction (SPE) method was employed to isolate the tested substances from the internal organs. Separation of the analytes was performed on a reversed-phase XTerra RP18 analytical column (3×150 mm, particle size 3.5 µm) using a gradient, the mobile phase consisted of a mixture acetonitril and 0.1% TFA in water. The total run cycle was 25 min. The flow rate was 0.4 ml/min, and injection volume was 10 µl. The excitation wavelength was set at 230 nm and emission wavelength at 336 nm. Excitation wavelength of internal standard (fluoresceine) was set at 440 nm and emission wavelength at 514 nm. Experiment demonstrated that highest concentration of ibogaine and noribogaine have been obtained in spleen and the lower one – in muscle of the animal after the direct administration into the mice stomach. Key words: Tabernanthe Iboga, ibogaine, noribogaine, solid phase extraction, distribution in mice organs, HPLC.