Cultivation of poliomyelitis virus in tissue culture. V. Observations on virus propagation in certain animal tissues with a synthetic nutrient medium.

Abstract

Further observations have been made on the propagation of Lansing poliomyelitis virus in tissue cultures. It has been observed that tissues derived from several organs of rhesus monkeys will support virus growth in tissue cultures in Erlenmeyer flasks with a synthetic medium as the source of nutrient. Cultures of tissues from monkey testis, lung, kidney, and gut have survived for long periods, and virus has been regularly recovered even from fluids removed from cultures after as late as 125 days. Cultures of tissues from monkey brain and cord, and muscle, did not survive as long, and less virus was demonstrated in the supernatant fluids. Muscle from the diaphragm did not appear to support growth. Cultures of tissues from the brain, kidney, and lung of beef embryos survived for long periods, but no virus was found in any of the culture fluids.