Abstract
Background. Mushrooms are increasingly becoming an important component of diets worldwide, and it is of paramount importance to choose appropriate substrates to grow them. The objective of this study was to grow Pleurotus ostreatus mushroom using different agricultural substrates. Methods. Corncobs, finger millet straw, and bamboo waste were collected from different sites of the Awi Zone. The substrates were chopped into small pieces, and 500 g of their dry mass alone and their combination was measured, packed in a polythene bag, moistened, and pasteurized. The cooled substrates were inoculated with a spoon of P. ostreatus spawn brought from Debre Berhan University. The bags were placed in the growing room, and growth parameters were recorded continuously with environmental variables. The experimental setup was a complete randomized design, six treatments with three replicates. Results. The fastest spawn running phase of P. ostreatus was 28.71 ± 0.80 days, pinhead formation was 32.36 ± 0.26 days, and fruiting bodies’ formation was 5.19 ± 0.74 days after the pinhead was recorded on the corncob substrate. The highest fresh weight and biological efficiency with the significant statistical association were obtained from P. ostreatus grown on finger millet straw (253.07 ± 1.05 and 50.20 ± 0.47, respectively). The highest average number of pinheads and fruiting bodies (29.60 and 11.44, respectively) was recorded on finger millet straw. The lowest biological efficiency (20.80 ± 0.41), fresh weight (101.48 ± 0.91), number of pinheads (14.40), and number of fruiting bodies (4.25) were recorded from a mixture of corncob and bamboo waste (50% each) substrates. Conclusion. Finger millet straw is recommended as the best substrate for the cultivation of P. ostreatus. The mixed substrate of corncob and bamboo waste (1 : 1) for P. ostreatus cultivation is not encouraged due to poor growth performance.
Highlights
Mushrooms are increasingly becoming an important component of diets worldwide, and it is of paramount importance to choose appropriate substrates to grow them. e objective of this study was to grow Pleurotus ostreatus mushroom using different agricultural substrates
Teff straw, paper waste, finger millet husk, and banana pseudo-stems are additional substrates used for the cultivation of oyster mushrooms [14,15,16,17]. erefore, the current study intended to evaluate Pleurotus ostreatus cultivation on different substrates alone and their combination
One-way ANOVA techniques were employed to test the overall significance of data in contrast to the least significance difference (LSD) test which compared the differences among variety means. e time taken to complete the growth of the mycelium on substrates, the appearance of pinheads, fresh weight, biological efficiency, and maturation of fruiting bodies of different treatments were compared
Summary
One of the world’s biggest challenges is food insecurity. is problem is largely common in low- and middle-income countries that mainly have poor food production systems and suffer from serious malnutrition. E genus Pleurotus consists of 40 different species commonly known as “oyster mushroom.”. Oyster mushroom production is becoming popular worldwide due to its potential to grow at a wide range of temperatures and utilization of various lignocelluloses [6]. Pleurotus species are widely cultivated worldwide, commonly in Asia, America, and Europe, because of their simpleness, low-cost production technology, and high biological efficiency [8]. E growth of the oyster mushroom requires high humidity (80–90%) and a temperature of 25–30°C for fruiting body formation [9]. E substrates that have been used for mushroom production in previous studies include rice straw, rice bran, wheat straw, pulp, corncobs, cocoa shell waste, cotton waste, spent grain, sawdust, maize husks, and cassava peelings [2, 11]. Teff straw, paper waste, finger millet husk, and banana pseudo-stems are additional substrates used for the cultivation of oyster mushrooms [14,15,16,17]. erefore, the current study intended to evaluate Pleurotus ostreatus cultivation on different substrates alone and their combination
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