Cultivation of Isolated Tissue derived from the Pericycle of Roots

Abstract

IN the growing root the pericycle is the site of the interesting morphogenetic activity, the initiation of lateral root primordia. This tissue also participates in the initiation of vascular cambium, which leads to the production of the secondary tissues of the root. For the investigation of the factors which initiate these activities in the pericycle and lead to the formation of organized tissues such as root primordia or cambium, it is desirable to have pericycle tissue isolated from the morphogenetic influence of neighbouring tissues. Surgical isolation would be almost impossible because of the small number of cells involved and the risk of injury.