Abstract
Bluetongue (BT) is a Culicoides vector borne disease of domestic and wild ruminants. It is caused by bluetongue virus (BTV). BT is infectious but non-contagious disease. The head–thorax region of Culicoides Oxystoma vector trapped on animal farm in TN was triturated and inoculated in BHK- 21 cell culture. After few blind passages it showed cytopathic effect (CPE) in cell culture. After appearance of 75% cytopathic effect in BHK 21 cell culture, viral nucleic acid was extracted. The RNA-PAGE analysis showed BTV specific characteristics migration pattern of 3:3:3:1. Viral nucleic acid was allowed for cDNA synthesis followed by NS1 and VP2 gene based RT-PCR. The NS1 gene RT-PCR, CPE and specific migration pattern in RNA-PAGE confirmed the sample as BTV. The VP2 gene based serotype specific RT-PCR identified the isolate as BTV serotype 16. The study suggested that Culicoides oxystoma could be a potential vector for transmission of BTV in southern India.
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