Abstract
The acceptor stem of tRNA(Ala) from E. coli has been chemically synthesized and crystallized. This duplex contains a G.U base pair in position 3-70, which is the main identity element for alanyl-tRNA synthetase from E. coli. The crystals are stable in the X-ray beam for a long period of time and diffract to 1.7 A resolution. The monoclinic crystals reveal a C2 space group with a = 35.0, b = 47.5, c = 26.2 A, beta = 102.3 degrees and one acceptor stem per asymmetric unit.
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Schedule a callMore From: Acta crystallographica. Section D, Biological crystallography
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