Abstract
Three carbapenem-resistant Escherichia coli were recovered from rectal swabs of different patients in a tertiary hospital and were found carrying blaNDM-4, an uncommon blaNDM variant. Genome sequences of the isolates were obtained using Illumina technology and the long-read MinION sequencer. The isolates belonged to ST405 and phylogenetic group D, a globally distributed lineage associated with antimicrobial resistance. In addition to blaNDM-4, the three isolates carried 14 known resistance genes including the extended-spectrum β-lactamase gene blaCTX-M-15. There were only 1 or 2 SNPs between the isolates, suggesting a common origin and cryptic transmission in hospital. blaNDM-4 was located on a 46.5-kb IncFIA self-transmissible plasmid, which may facilitate further dissemination of blaNDM-4. Two copies of IS26 bracketed a 14.6-kb region containing blaNDM-4 and have the potential to form a composite transposon for mediating the mobilization of blaNDM-4.
Highlights
Carbapenem-resistant Enterobacteriaceae (CRE) have emerged as a major challenge to global public health
The three isolates had blaNDM only, which was identified as blaNDM-4 by amplifying and sequencing the complete coding sequence of blaNDM using additional primers[4]
The three isolates belonged to ST405 and phylogenetic group D
Summary
Carbapenem-resistant Enterobacteriaceae (CRE) have emerged as a major challenge to global public health. Antimicrobial resistance genes were identified from genome sequences using the ABRicate (https://github.com/tseemann/abricate) program. Draft whole-genome sequences of isolates WCHEC1837, WCHEC96200 and WCHEC99540 have been deposited into GenBank under the accession numbers NGUU00000000, NGUV00000000 and NGUW00000000, respectively.
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