Cryopreservation of herbaceous Asteraceae seeds: Effects of seed reserves on seed germination and seedling regrowth

Abstract

Carbohydrate, lipid and protein, the three main storage reserves of seeds, are the main sources of raw materials and energy for seed germination and early growth stage of seedlings. In this study, the seed of 16 Asteraceae herbaceous ornamentals were used to compare the changes in seed germination, seedling growth indexes and four major storage reserves of seeds before and after cryopreservation. After cryopreservation, seed germination and seedling growth were preserved in most species (11/16), while one species showed significantly improved post-thaw germination and four had decreased germination. In seeds with preserved germination, no clear variation was noted with regards to the four kinds of storage reserves, or only a slight change in one. However, significantly reduced soluble protein, soluble sugar and fat content and increased starch content were detected in seeds showing reduced germination after cryopreservation. The correlation study further showed that the changes in seed germination were positively correlated with soluble protein, soluble sugar and fat, and negatively correlated with starch. In seedling growth indexes, only cotyledon area was correlated to the content of soluble protein, starch, fat, and seed germination after cryopreservation. These results support the use of soluble protein, soluble sugar, starch and fat as indicators of seed germination after cryopreservation, but not for predicting the seedling growth indexes.