Crude black pepper phytochemical 3D printed cell based miniaturized hydrazine electrochemical sensing platform

Abstract

Abstract Herein, a glassy carbon electrode (GCE) modified with a hybrid of crude black pepper-graphitized mesoporous carbon (GCE/GMC@pep) for an electrochemical detection of hydrazine is presented. The GCE/GMC@pep shows a surface-confined redox peak at an electrode potential E1/2 = + 235 mV vs.Ag/AgCl in pH 7 PBS with a surface excess value Ʈe-pep = 13.19 × 10−9 mol−1 cm2. The physico-chemical characterization unveils the interaction of GMC with piperine. Further, GCE/GMC@pep manifests a highly selective electro-catalytic activity towards Hydrazine in neutral pH at +257 mV in a linear detection range of 70 μM-7 mM with lower limit of detection of 40 μM(with cyclic voltammetry) and 30 μM-5 mM with lower limit of detection of 0.5 μM (with chronoamperometry). More so, the fabricated electrode system provides an insignificant interference from co-existing biochemical / chemicals. Real samples, tobacco, cigarette smoke, lake water and human blood serum, are further analyzed via standard addition approach. Overall, this study describes a prototype module for tuning the redox active behavior of selective phytochemical for electrochemical sensing applications.