Abstract
PurposeThis study aimed to explore the effects of genetic polymorphisms in metabolic enzymes on relative telomere length changes and explore the mechanism of canceration induced by omethoate.Materials and Methods180 long-term omethoate-exposed workers and 115 healthy controls were recruited. Real-time PCR method was applied to determine the relative telomere length in peripheral blood leukocytes DNA, and Six polymorphic loci of GSTT1(+/−), GSTM1(+/−), GSTP1 rs1695, CYP2E1 rs6413432, CYP2E1 rs3813867 and PON2 rs12026 were detected by polymerase chain reaction and restriction fragment length polymorphism method; Multiple linear regression was conducted to explore the effects of omethoate exposure and genetic polymorphisms on the telomere length.ResultsThe relative telomere lengths in the control group (0.94 [0.76, 1.32]) were significantly shorter than that in the exposure group (1.50 [1.11, 2.57]) (Z = 7.910, P < 0.001). Univariate analysis showed that the relative telomere lengths of the GSTM1-deletion individuals were significantly longer than that of the non - deletion genotype in the control group (Z = 2.911, P = 0.004), and the relative telomere lengths of GSTP1 rs1695 polymorphism locus (GG+AG) genotype individuals were longer than that of AA genotype in the exposure group. The difference was statistically significant (Z = 2.262, P = 0.024). Multivariate analysis found that pesticide-exposure (b = 0.524, P < 0.001) and GSTM1 polymorphism (b = −0.136, P = 0.029) had an impact on telomere length.ConclusionsThe relative telomere lengths of omethoate-exposure workers were longer than that in the control population. Also GSTM1 genetic polymorphism may influence the changes of the telomere length induced by omethoate.
Highlights
Omethoate is an organophosphorous insecticide and acaricide that is highly toxic, highly effective and broadspectrum features
Univariate analysis showed that the relative telomere lengths of the GSTM1-deletion individuals were significantly longer than that of the non - deletion genotype in the control group (Z = 2.911, P = 0.004), and the relative telomere lengths of GSTP1 rs1695 polymorphism locus (GG+AG) genotype individuals were longer than that of AA genotype in the exposure group
GSTM1 genetic polymorphism may influence the changes of the telomere length induced by omethoate
Summary
Omethoate is an organophosphorous insecticide and acaricide that is highly toxic, highly effective and broadspectrum features. Acute organophosphorous poisoning incidents have been gradually reduced, and people are www.impactjournals.com/oncotarget more concerned about the chronic toxic effect caused by long-term, low-dose exposure to OPs. More and more studies have concluded that there was a close relationship between long-term, low-dose exposure Ops and many human diseases, such as tumorigenesis, adverse reproductive outcomes, and neurological and neurobehavioral function abnormalities [3,4,5]. Studies on organophosphorous pesticides involving genetic damage mainly included comet assay, micronucles (MN), and sister chromatid exchange (SCE) [6,7,8,9,10,11]. It was rarely reported that OPs induced telomere damage. Telomere length can be used as an effect marker for analysis and research
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