Abstract
ABSTRACT: Leporinus friderici is a migratory neotropical fish with elevated ecological and economic importance in Brazil. Microsatellite markers are highly important in population genetic studies, management, and conservation programs; however, no markers are available for this species. In this study, seven microsatellite loci, previously developed for Megaleporinus obtusidens, were successfully cross-amplified in L. friderici. Among these loci, five presented moderate to high genetic variability levels, with four to seven alleles per loci and expected heterozygosities varying from ≥ 0.574 to 1.000. These markers represent a valuable tool for the future management and ecological studies involving this species and group of neotropical fishes.
Highlights
RESUMO: Leporinus friderici é um peixe neotropical migratório com elevada importância ecológica e econômica no Brasil
Popularly known as “piau-trêspintas”, naturally occurs in Suriname, the Amazon, Paraná and Paraguay River Basins (GODOY, 1987). This species plays an important ecological role in its ecosystem, and has social and economic importance for local fisheries (OLIVATTI et al, 2011). Their natural populations are declining in response to several anthropogenic disturbances, such as habitat modifications, water pollution, construction of dams, and the absence of adequate conservation practices (ABELL et al, 2008; BARLETTA et al, 2010)
Microsatellite markers are intensively used in population genetic studies, mainly due to their wide distribution along the eukaryotic genome, high levels of polymorphism, codominance, and high reproducibility (CHISTIAKOV et al, 2006)
Summary
RESUMO: Leporinus friderici é um peixe neotropical migratório com elevada importância ecológica e econômica no Brasil. The development and standardization of molecular markers, such as microsatellites, are cornerstones for the conservation of natural resources (ALLENDORF et al, 2017) and the implementation of adequate and sustainable management in aquaculture. The cross-species amplification of 15 microsatellite loci, previously developed for Megaleporinus
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