Cross-Linking of Escherichia coli Formamidopyrymidine-DNA Glycosylase to DNA Duplexes Containing Photoactivatable Phenyl(Trifluoromethyl)diazirine Groups

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New reactive analogs of substrates for DNA repair enzyme E. coli Fpg protein containing the residues of 8-oxoguanine and photoactivatable phenyl(trifluoromethyl)diazirine groups were synthesized. Their substrate properties were investigated. Using photocross-linking technique, we established the presence of contacts of two nucleosides located near the oxoG with amino acids from the Fpg protein. The cross-linking efficiency achieved 10%.