Abstract
The temperate and herbaceous genus Vicia L. is a member of the legume tribe Fabeae of the subfamily Papilionoideae. The genus Vicia comprises 166 annual or perennial species distributed mainly in Europe, Asia, and North America, but also extending to the temperate regions of South America and tropical Africa. The use of simple sequence repeat (SSR) markers for Vicia species has not been investigated as extensively as for other crop species. In this study, we assessed the potential for cross-species amplification of cDNA microsatellite markers developed from common vetch (Vicia sativa subsp. sativa). For cross-species amplification of the SSRs, amplification was carried out with genomic DNA isolated from two to eight accessions of 22 different Vicia species. For individual species or subspecies, the transferability rates ranged from 33% for V. ervilia to 82% for V. sativa subsp. nigra with an average rate of 52.0%. Because the rate of successful SSR marker amplification generally correlates with genetic distance, these SSR markers are potentially useful for analyzing genetic relationships between or within Vicia species.
Highlights
The genus Vicia L. is a member of the legume tribe Fabeae of the subfamily Papilionoideae [1]
We examined the transferability of simple sequence repeat (SSR) microsatellite markers from the V. sativa genome to distantly related Vicia species
36 V. sativa cDNA-derived SSR primer pairs were tested for their ability to cross-amplify across 22 other Vicia species
Summary
The genus Vicia L. is a member of the legume tribe Fabeae (sometimes referred to as “Vicieae”) of the subfamily Papilionoideae [1]. The development of DNA sequence-based molecular markers is expensive To overcome this limitation, transferability of molecular markers among species has been studied [9,10,11]. Simple sequence repeat (SSR) markers are considered to be efficient due to their co-dominant and multi-allelic nature, relative abundance, high genome coverage, and reproducibility of results [12]. Due to the conserved nature of transcribed regions, EST-based markers are considered to be important because of their high rate of transferability across species [14]. Our findings provide information on the rates of cross-species transferability of SSRs, and the relative degree of SSR polymorphism that will allow the establishment of more consistent relationships among the Vicia species
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