Abstract
CRISPOR.org is a web tool for genome editing experiments with the CRISPR–Cas9 system. It finds guide RNAs in an input sequence and ranks them according to different scores that evaluate potential off-targets in the genome of interest and predict on-target activity. The list of genomes is continuously expanded, with more 150 genomes added in the last two years. CRISPOR tries to provide a comprehensive solution from selection, cloning and expression of guide RNA as well as providing primers needed for testing guide activity and potential off-targets. Recent developments include batch design for genome-wide CRISPR and saturation screens, creating custom oligonucleotides for guide cloning and the design of next generation sequencing primers to test for off-target mutations. CRISPOR is available from http://crispor.org, including the full source code of the website and a stand-alone, command-line version.
Highlights
Genome editing with the CRISPR–Cas system is impacting all fields of life sciences
We initially developed CRISPOR to help with guide RNA selection in genomes from non-conventional model organisms for which no tools were available yet
While we were developing the website, many design criteria were published, so we compared them, published the comparison [4] and designed CRISPOR such that it recommends scores that are most accurate for a particular assay
Summary
Genome editing with the CRISPR–Cas system is impacting all fields of life sciences. While the technique is comparatively simple, cleavage specificity and efficiency depend primarily on the choice of the CRISPR guide sequence. To help with this step, various web tools find potential guide RNAs in an input sequence and rank them according to off-target and on-target predictions [1,2,3]. We initially developed CRISPOR to help with guide RNA selection in genomes from non-conventional model organisms for which no tools were available yet.
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