Creole bean seeds microbiolization with doses of Trichoderma harzianum

Laís Dalzotto,Paola Mendes Milanesi,Vanessa Neumann Silva,Francine Spitza Stefanski,Brenda Tortelli,Deivid Sacon

doi:10.1590/0103-8478cr20190542

Laís Dalzotto, Paola Mendes Milanesi + Show 4 more

Open Access

https://doi.org/10.1590/0103-8478cr20190542

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Abstract

ABSTRACT: In the search for improved yields, seed treatment by microbiolization has been used as an alternative to chemical treatment. The objective was to verify the physiological and sanitary quality of creole bean seeds, var. Chumbinho, after microbiolization with doses of a commercial product (c.p.) with Trichoderma harzianum (strain ESALQ-1306). The treatments were: T1) 100 mL c.p./100 kg seeds; T2) 150 mL c.p.; T3) 200 mL c.p.; T4) 200 mL of chemical treatment (c.p., 250 g L-1 fipronil + 25 g L-1 pyraclostrobin + 225 g L-1 thiophanate-methyl); and T5) control (without coating of seeds). The tests were: sanitary test (blotter test); germination and first count; accelerated aging, cold germination without soil, speed of germination rate (SGR), seedling shoot and root lengths, and emergence of seedlings in a greenhouse. T. harzianum controlled Aspergillus spp., Penicillium spp. and Fusarium oxysporum. With 100 mL c.p. of T. harzianum dose had better results for the germination and vigor, and this dose it is an alternative to chemical treatment in creole bean seeds.

Highlights

The common bean (Phaseolus vulgaris L.), belonging to the family Fabaceae, is a common food on the tables of Brazilians, being a source of vegetable protein
Trichoderma harzianum, derived from the commercial product used to treat the seeds, grew, though it was less evident in the chemical treatment
Treatments with Trichoderma harzianum (100, 150 and 200 mL) and the chemical treatment did not differ statistically from the control, though they presented with a lower incidence of Aspergillus spp. and Macrophomina phaseolina on the creole bean seeds (Table 1), or because the isolate of Trichoderma spp. probably there was no antagonistic control of this fungi (MIGLIORINI et al, 2012)

Summary

Introduction

The common bean (Phaseolus vulgaris L.), belonging to the family Fabaceae, is a common food on the tables of Brazilians, being a source of vegetable protein. Microbiolization has been used as an alternative to chemical treatment, consisting of the application of beneficial microorganisms (e.g. Trichoderma spp.) to seeds in order to control phytopathogens (MACHADO et al, 2012). Species of the genus Trichoderma are freeliving fungi that interact with soil, roots and leaves They are widely used in agricultural crops because of their high reproductive capacity and ability to survive under unfavorable conditions, contributing to the stimulation of defense mechanisms against pathogenic fungi (HARMAN, 2000)

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