Creation of seed breeding material of seed oats (Avena sativa L.) using embryo culture

Abstract

The use of somaclonal seed oat samples in the breeding process allows to significantly expand the genetic variability of the experimental material. Callus was obtained by embryo culture from mature oat embryos sown on MS nutrient medium from 32.6% in No. 471–1117 with a concentration of 4 mg/l 2,4-D to 97.33% in No. 583–35 with a concentration of 2 mg/l. The regeneration ability of calli from different mature embryos of seeded oat offspring obtained on a nutrient medium with a concentration of 2,4-D in an induction medium of 2 mg/l ranged from 9.87 ± 2.42 in membranous offspring 479–1342 to 14.63 ± 5.52% in whole grains. As a result, regeneration lines of oat for obtaining seeds and further use in the breeding process were created.