CREATION AND PROPERTIES OF BIOCOMPOSITE NANOPARTICLES BASED ON FUCOIDAN AS CARRIERS OF FIBRINOLYTIC ENZYME

Abstract

Magnetically guided biodegradable nanocomposites are a new class of functional materials that expand the possibilities of clinical therapy. The paper describes the chemistry of the formation of a nanobiocomposite material based on a natural polymer synthesized by electrostatic interaction of a polysaccharide and magnetite in an aqueous medium. The possibility of electrostatic immobilization of a model fibrinolytic enzyme was studied. An increase in the electrochemical potential of particles during immobilization of a fibrinolytic enzyme was established by the method of dynamic light scattering. The degree of enzyme inclusion was 9.95±0.05 mass%. The average particle size of the nanobiocomposite material after immobilization of the enzyme was 264.3 nm. The study of the magnetic properties showed the absence of residual magnetization, which makes it possible to exclude the possibility of magnetic aggregation when using the biocomposite in a biological application. The saturation magnetization of the formed material was 57.1 ± 2.3 kA/m, which is lower than that of pure magnetite sol (MS ∼ 73.8 ± 3.4 kA/m), but sufficient for manipulation under conditions of resistance to blood flow. The kinetics of the release of an enzyme preparation in a phosphate buffer from a nanocomposite carrier based on fucoidan was studied. To study the kinetics of enzyme release by mathematical modeling, zero and first order models, the Korsmeier-Peppas model, the Higuchi model, and the Baker-Lonsdale model were used. It has been established that the kinetics of fibrinolytic enzyme release is described with high accuracy (r2 = 0.98) by the Korsmeier–Peppas equation, where the process of release of the enzyme preparation is influenced by diffusion obeying Fick's law. It has been established that zero and first order models are applicable only to the initial (0-40 min, where r2 = 0.96) and final (80-320 min, r2 = 0.93) stage of release.