Abstract
Introduction: Recent technological advancements in single-cell RNA sequencing and single-cell quantitative real-time PCR have enabled researchers to conduct single-cell analyses of limbal epithelial cells. Aim of the study: the main aim of the study is to Cultivating corneal stem cells with a thermoreversible gelation polymer Material and method: Each human limbal biopsy were placed in 1 mL of TC medium containing 3% FCS, antibiotics (penicillin, gentamicin, and amphotericin B), and DMEM, and then transported to a cell biology lab. Conclusion: The thermoreversible gelation polymer facilitates the growth of limbal epithelial cells. The limbal characteristic could still be seen in the grown cells
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