Abstract
BackgroundThe objective of this study was to place bone graft materials in cranial defects in a rabbit model and compare their bone regenerating ability according to the size and density of demineralized dentin matrix (DDM).MethodsWe selected nine healthy male rabbits that were raised under the same conditions and that weighed about 3 kg. Two circular defects 8 mm in diameter were created in each side of the cranium. The defects were grafted with DDM using four different particle sizes and densities: 0.1 mL of 0.25- to 1.0-mm particles (group 1); 0.2 mL of 0.25- to 1.0-mm particles (group 2); 0.1 mL of 1.0- to 2.0-mm particles (group 3); and 0.2 mL of 1.0- to 2.0-mm particles (group 4). After 2, 4, and 8 weeks, the rabbits were sacrificed, and bone samples were evaluated by means of histologic, histomorphometric, and quantitative RT-PCR analysis.ResultsIn group 1, osteoblast activity and bone formation were greater than in the other three groups on histological examination. In groups 2, 3, and 4, dense connective tissue was seen around original bone even after 8 weeks. Histomorphometric analysis of representative sections in group 1 showed a higher rate of new bone formation, but the difference from the other groups was not statistically significant. RT-PCR analysis indicated a correlation between bone formation and protein (osteonectin and osteopontin) expression.ConclusionsDDM with a space between particles of 200 μm was effective in bone formation, suggesting that materials with a small particle size could reasonably be used for bone grafting.
Highlights
The objective of this study was to place bone graft materials in cranial defects in a rabbit model and compare their bone regenerating ability according to the size and density of demineralized dentin matrix (DDM)
Bone graft materials can be classified based on their origin, that is, autogenic, allogenic, xenogenic, and alloplastic
The new bone had formed from the existing bone, it had not formed between the materials (Fig. 3g, h)
Summary
The objective of this study was to place bone graft materials in cranial defects in a rabbit model and compare their bone regenerating ability according to the size and density of demineralized dentin matrix (DDM). Dental implants have been used to restore defects in patients who are partially or completely edentulous. The proper placement of implants and adequate bone are necessary for long-term stability and sufficient functionality. Dental implantation was possible under only limited circumstances; with improvements in the implant itself, as well as in clinical techniques, bone grafting enables bone to form even when it is deficient, increasing the rate of success. Bone graft materials can be classified based on their origin, that is, autogenic, allogenic, xenogenic, and alloplastic [1]. Autogenic bone is considered to be the ideal material, it is limited in terms of its availability, the need for additional surgery, and complications [2,3,4,5]. Allogenic, xenogenic, and alloplastic types of the bone are used more often, even though allogenic and xenogenic bone are associated with such problems as immune reactions and infection, and alloplastic bone provides merely a frame for bone formation [6,7,8,9,10]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
