Abstract
Cow dung and agroresidues were used as the substrates for the production of alkaline proteases by Pseudomonas putida strain AT in solid-state fermentation. Among the various substrates evaluated, cow dung supported maximum (1351±217 U/g) protease production. The optimum conditions for the production of alkaline proteases were a fermentation period of 48 h, 120% (v/w) moisture, pH 9, and the addition of 6% (v/w) inoculum, 1.5% (w/w) trehalose, and 2.0% (w/w) yeast extract to the cow dung substrate. The enzyme was active over a range of temperatures (50–70°C) and pHs (8–10), with maximum activity at 60°C and pH 9. These enzymes showed stability towards surfactants, detergents, and solvent and digested various natural proteins.
Highlights
Proteases constitute one of the commercially important groups of extracellular microbial enzymes and their annual sales account for 60% of the total world enzyme market [1]
From an industrial point of view, it is estimated that around 30–40% of the production cost of industrial enzymes can be attributed to the cost of the growth medium [3]
A few reports were available on the use of cow dung for the production of alkaline proteases, for example, cow dung and Halomonas sp
Summary
Proteases constitute one of the commercially important groups of extracellular microbial enzymes and their annual sales account for 60% of the total world enzyme market [1]. Cow dung substrate (referred to as dungstrate hereafter) was used as a cheap substrate for the production of alkaline proteases for various biotechnological applications. A few reports were available on the use of cow dung for the production of alkaline proteases, for example, cow dung and Halomonas sp. There might hardly be any reports on the use of cow dung for the production of proteases by Pseudomonas sp. Even though Bacillus spp. have been viewed as a promising group of organisms for protease production for various industrial applications, Pseudomonas protease has been considered for many applications. Due to the importance of Pseudomonas proteases for various applications, cow dung could contribute significantly as a solid substrate in enzyme bioprocess and may help to reduce production costs
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