Abstract
Immerse pieces of brain tissue 4 wk in solutions A and B, mixed just before use: A. K2Cr2O7, 1 gm; HgCl2, 1 gm; boiling distilled water, 85 ml. Boil A for 15 min, cool to 2 C and add: B. K2CrO4, 0.8 gm; Na2WO4, 0.5 gm; distilled water, 20 ml. Rinse in water and immerse 24 hr in LiOH, 0.5 gm; KNO3, 15 gm; distilled water, 100 ml. Wash 24 hr in several changes of 0.2% acetic acid and then for 2 hr in tap water. Dehydrate and embed in celloidin. Process a 60 μ section through 70 and 95% ethanol, a 3:1 mixture of absolute ethanol and chloroform, and toluene. Immerse it for 5 min in a solution containing methyl benzoate, 25 ml; benzyl alcohol, 100 ml; chloroform, 75 ml. Orient the section on a chemically clean slide and let air-dry 5–10 min. Process through toluene, 3:1 ethanol-chloroform and 95% ethanol. Place the section for 5–60 min at 60 C in a solution made up of: Luxol fast blue G (Matheson, Coleman and Bell), 1 gm; 95% ethanol, 1000 ml; 10% acetic acid, 5 ml. Hydrate to water and immerse in 0.05% Li2CO3...
Published Version
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