Co-treatment Effect of Syzygium cumini (L.) Skeels on Indomethacin Induced Gastric Ulcer on Mice Model

Abstract

Syzygium cumini (L.) Skeels or SCC originally from India and Southeast Asia, commonly used as a medicinal and acted as antioxidant and anti-inflammatory plant. Indomethacin, which is a part of nonsteroidal anti-inflammatory drugs (NSAIDs) family, induced gastric damage and perforation through the excess generation of reactive oxygen species (ROS). This research focus on co-treatment administered between SCC and indomethacin in the subsequent 7 days and evaluated on oxidative damage, inflammatory parameter and epidermal growth factor (EGF) receptor. SCC showed a concentration and dose dependent reduction in ulcer index (UI) values leading to the increasing of inhibition percent when compared to indomethacin treated mice, confirmed by photographer which showed maximum efficacy on day 5 of treatment. On day 1 and day 3 of ulceration, malondialdehyde (MDA), oxidized glutathione (GSSG), nitrile contents and tumor necrosis factor-alpha (TNF-α) were increased. Gastric wall mucus and glutathione peroxidase (GPx) were down. After that gastric mucosa were recovered by healing processed and were regenerated the mucosal cap to promote EGF receptor. SCC was increased the up-regulation of COX-1 enzyme resulting in down-regulation to COX-2 expression at day 3 of ulceration. At day 5 and 7, the gastric ulceration were regenerated themselves. These all results indicated that SCC have a great protective effect against indomethacin induced gastric ulcer in in-vivo co-treatment model.