Cortical microtubule orientation in Arabidopsis thaliana root meristematic zone depends on cell division and requires severing by katanin

Emmanuel Panteris,Ioannis-Dimosthenis S Adamakis,Barbara-Evelin Diannelidis

doi:10.1186/s40709-018-0082-6

Emmanuel Panteris, Ioannis-Dimosthenis S Adamakis + Show 1 more

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BackgroundTransverse cortical microtubule orientation, critical for anisotropic cell expansion, is established in the meristematic root zone. Intending to elucidate the possible prerequisites for this establishment and factors that are involved, microtubule organization was studied in roots of Arabidopsis thaliana, wild-type and the p60-katanin mutants fra2, ktn1-2 and lue1. Transverse cortical microtubule orientation in the meristematic root zone has proven to persist under several regimes inhibiting root elongation. This persistence was attributed to the constant moderate elongation of meristematic cells, prior to mitotic division. Therefore, A. thaliana wild-type seedlings were treated with aphidicolin, in order to prevent mitosis and inhibit premitotic cell elongation.ResultsIn roots treated with aphidicolin for 12 h, cell divisions still occurred and microtubules were transverse. After 24 and 48 h of treatment, meristematic cell divisions and the prerequisite elongation ceased, while microtubule orientation became random. In meristematic cells of the p60-katanin mutants, apart from a general transverse microtubule pattern, cortical microtubules with random orientation were observed, also converging at several cortical sites, in contrast to the uniform transverse pattern of wild-type cells.ConclusionTaken together, these observations reveal that transverse cortical microtubule orientation in the meristematic zone of A. thaliana root is cell division-dependent and requires severing by katanin.

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Transverse cortical microtubule orientation, critical for anisotropic cell expansion, is established in the meristematic root zone
Taken together, these observations reveal that transverse cortical microtubule orientation in the meris‐ tematic zone of A. thaliana root is cell division-dependent and requires severing by katanin
In all the developmental root zones, anisotropic cell expansion requires transversely oriented cellulose microfibrils in the cell wall, following the arrangement of cortical microtubules [2,3,4,5]. It has been confirmed in Arabidopsis thaliana that transverse cortical microtubule orientation is initially established in

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Transverse cortical microtubule orientation, critical for anisotropic cell expansion, is established in the meristematic root zone. In all the developmental root zones, anisotropic cell expansion requires transversely oriented cellulose microfibrils in the cell wall, following the arrangement of cortical microtubules [2,3,4,5]. It has been confirmed in Arabidopsis thaliana that transverse cortical microtubule orientation is initially established in. To interpret this difference in cortical microtubule orientation persistence, it was suggested that neither genetic nor chemically-induced inhibition of cellulose synthesis and/or conformation may disrupt the cell cycle in meristematic cells. Cell expansion prior to cell division perseveres and is a critical factor for establishing and maintaining transverse microtubule orientation in meristematic root cells [4, 5].

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