Corrigendum

Abstract

Journal of Comparative NeurologyVolume 412, Issue 4 p. 708-708 CorrigendumFree Access Corrigendum First published: 26 August 1999 https://doi.org/10.1002/(SICI)1096-9861(19991004)412:4<708::AID-CNE12>3.0.CO;2-7Citations: 8AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract In a previous manuscript the staining pattern of a monoclonal antibody generated to the N-terminal sequence of the ORL-1 receptor was described in the rat CNS (Anton et al., 1996). Subsequently, this antibody was used in double-label confocal experiments to map mu opioid and ORL-1 receptors in areas of brain associated with pain processing (Monteillet-Agius et al., 1998). In order to stain sections in mice, the monoclonal antibody was biotinylated, and as one of the specificity controls, brain sections from mutant mice lacking the ORL-1 receptor were stained. Mice null for both exon 2 and 3 of the ORL-1 receptor (generated by Zhenping Chen in John Pintar's laboratory, UMDNJ-Robert Wood Johnson Medical School, unpublished) as well as exon 1 knockout mice, which eliminates the epitope to which the antibody was raised, (Nishi et al., 1997) were tested and the same results obtained. The biotinylated antibody showed a staining pattern in the ORL-1 knockout mice indistinguishable from wild-type mice and similar to the pattern found in rat brain. Although the CNS staining pattern in rat was consistent with the distribution of mRNA for the receptor, the retained staining pattern in ORL-1 null mice demonstrates that the monoclonal antibody is recognizing a protein distinct from the ORL-1 receptor. LITERATURE CITED Anton B, Fein J, To T, Li X, Silberstein L, Evans CJ. 1996. Immunohistochemical localization of ORL-1 in the central nervous system of the rat. J Comp Neurol 368: 229– 251. Medline Monteillet-Agius G, Fein J, Anton B, Evans CJ. 1998. ORL-1 and mu opioid receptor antisera label different fibers in areas involved in pain processing. J Comp Neurol 399: 373– 383. Medline Nishi M, Houtani T, Noda Y, Mamiya T, Sato K, Doi T, Kuno J, Takeshima H, Nukada T, Nabeshima T, Yamashita T, Noda T, Sugimoto T. 1997. Unrestrained nociceptive response and disregulation of hearing ability in mice lacking the nociceptin/orphaninFQ receptor. EMBO J 16: 1858– 1864. Medline Citing Literature Volume412, Issue44 October 1999Pages 708-708 ReferencesRelatedInformation