Correlative Study on Serum Levels of Zinc, Interleukin-2, -10 of Patients With Cervical Human Papilloma Virus-16 Infection

Abstract

Objective To explore changes and significance of serum levels of Zinc (Zn), interleukin (IL)-2, -10 of patients with cervical human papilloma virus (HPV)-16 infection. Methods From March 2009 to March 2010, 73 patients with cervical lesions and high-risk human papilloma virus (HR-HPV)-16 infection were recruited into this study, including 19 cases of cervical intraepithelial neoplasia (CIN)Ⅰ(CINⅠgroup), 21 of CIN Ⅱ(CIN Ⅱ group), 18 of CIN Ⅲ (CIN Ⅲ group)and 15 of cervical cancer(cervical cancer group). Meanwhile, 30 patients with chronic cervicitis without human papilloma virus infection were selected into control group. The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Second Hospital of Tianjin Medical University. Informed consent was obtained from all participants. The serum zinc content was detected by atomic absorption spectroscopy (AAS), and the serum content of interleukin-2, -10 were detected by the method of ELISA. Ages, history of other autoimmune diseases, cancer and other medical disease, had no significant differences among 5 groups (P>0.05). Results ①There had no significant differences of serum zinc, interleukin-2 among CIN Ⅰ, CIN Ⅱ and cervical group (P 0.05). There had significant differences of serum interleukin-10 among CIN Ⅰ, CIN Ⅱ and control group (P>0.05). There had no significant differences of interleukin-10 between CIN Ⅰ and control group (P>0.05). There had significant differences of interleukin-10 between CIN Ⅲ and CIN Ⅱ group (P<0.05). ②Positive correlation were found between serum znic content and interleukin-2 (r=0.69, P=0.00). There had no positive correlation between serum content of interleukin-10 and znic (r=0.19, P=0.05). Conclusion Th1/Th2 shift in high-risk human papilloma virus infected patients with high-grade cervical lesions. Znic deficiency may be involved in this change. Key words: znic (Zn); human papilloma virus (HPV); interleukin (IL); Th1/Th2