CorrelationShip Between Complication of Diabetes And Biofilm Form By Bacterial Isolate Isolated From Diabetic Foot Ulcer (DFU)Among Iraqi Diabetic Patients

Abstract

Background:Diabetic Foot ulcer (DFU)are the most common economicproblems in diabetic patients (both type І and ІІ), Sothat’s more severe and longer cure than non-diabetic patients, that has many degree of ulcer as mild, moderate, or severewhich maybe leading to development of gangreneand it is characterized by many important pathological complications as Hypertension; Retinopathy; Nephropathy and Neuropathy.Objectives –Biofilm form by Bacterial isolate isolated from DFUandstudy their correlated with many important factors as gender; age; type of Diabetic; Duration of ulcer and complication of diabetes such as Hypertension; Retinopathy; Nephropathy and Neuropathy.Material and methods –From 72 diabetic patients with DFUwho attended the canter forEndocrinology and Diabetes were collected swab samples ,used classical conventional techniques to culture all sample and Gram staining followed diagnostic by Vitek system, so biofilm detection by microtiter plate (Mtp), as well as the complication of diabetic were diagnosis by specialized physician.Results –Total of diabetic foot patients were males dominant over (73.3% ) than female (26.3%) , second age group of 41- 60 years were most prevalence (54.4%) whilst (21%) in third age group (>61),So produced biofilm high percentage (54.4%) in male ,but (15.8%) in female as well as the second age group (41-60 ) have high form biofilm (55%) whereas both the first and third age group ( < 41 and > 61) have low percentage (22.5%), andtype ІІ more than type І in both duration (>10 year and < 10 year ) as ( 49 , 24.6 %) respectively in type ІІ ,and ( 8.8 , 17.5 %) respectively in type І , and biofilm formation was (15.8 %) of the bacteria isolated DFUof patients with diabetes type І whilst ( 54.1%) in type ІІ ,so biofilm were formed predominantly by diabetic patients with Nephropathy (30 % ) followed diabetic patients with Hypertension (28 % ) and Neuropathy ( 24.5% ) , whilst only ( 17.5 % ) with retinopathy , Soboth E.col and Klebsiella pneumoniae to be equally responsible for diabetic foot ulcer as percentage (20%) successive followed by Staphylococcus aureus (14% ) and Pseudomonas aeruginosa(10% ).Conclusion –Diabetic foot ulcer (DFU) in males dominant over females also biofilm produced in male more than female and maximum number of DFU belonged to second age group of 41- 60 years,so second age group have high form biofilm than others, as well as type ІІ more than type І in both duration (>10 year and < 10 year),as well as Biofilm form by bacteria isolated from DFU of patients with diabetes type І more than type ІІ.Biofilms formed predominantly by diabetic patients with Nephropathy followed diabetic patients with Hypertension and Neuropathy as well as both E.col and Klebsiella pneumoniae to be equally responsible for diabetic foot ulcer.