Correlation of peroxisome proliferation-activated receptor in pituitary adenoma

Abstract

Objective To prove the expression of PPARγ in human pituitary adenomas,and administrate that the expression among different tumors has significant difference.Method 83 cases of pituitary adenoma tissues and 6 normal pituitary tissues were divided into different groups by immunohischemieal according to the source of tissues and cells.The mRNA of PPARγ in 83 pituitary adenomas tissues and 6 normal pituitary tissues were determined by RT-PCR and quantitative real-time PCR,the protein of PPARγ were detected by western-blot.Result we have 89 samples,17 samples tested as GH secreting-adenomas,15 samples tested as PRL secreting-adenomas,18 samples tested as ACTH secreting-adenomas,17 samples tested as miscellaneous pituitary adenomas (MCPAs),16 samples tested as nonfunctional pituitary adenomas (NFAs) and 6 samples tested as normal;the PPARγ mRNA expressions were higher in all kinds of pituitary adenomas than in the normal pituitary tissue.GH secreting-adenomas was the highest,and the differentia was significant in statistics;the protein expression level of PPARγ in GH secreting,Prolactin secreting,ATCH secreting and MCPAs were higher than in the normal pituitary tissue (P＜0.05),and the expression in GH secreting-adenomas was the highest which has markedly differentia.The expression level of NFAs has no different compared with normal pituitary (P＞0.05).Conclusion We supposed that PPARγ is an important molecular target in pituitary adenoma cells,especially for GH secreting-adenomas. Key words: PPARγ; Pituitary adenoma; GH secreting-adenoma; Nonfunctioning pituitary adenoma