Abstract
Objective To prove the expression of PPARγ in human pituitary adenomas,and administrate that the expression among different tumors has significant difference.Method 83 cases of pituitary adenoma tissues and 6 normal pituitary tissues were divided into different groups by immunohischemieal according to the source of tissues and cells.The mRNA of PPARγ in 83 pituitary adenomas tissues and 6 normal pituitary tissues were determined by RT-PCR and quantitative real-time PCR,the protein of PPARγ were detected by western-blot.Result we have 89 samples,17 samples tested as GH secreting-adenomas,15 samples tested as PRL secreting-adenomas,18 samples tested as ACTH secreting-adenomas,17 samples tested as miscellaneous pituitary adenomas (MCPAs),16 samples tested as nonfunctional pituitary adenomas (NFAs) and 6 samples tested as normal;the PPARγ mRNA expressions were higher in all kinds of pituitary adenomas than in the normal pituitary tissue.GH secreting-adenomas was the highest,and the differentia was significant in statistics;the protein expression level of PPARγ in GH secreting,Prolactin secreting,ATCH secreting and MCPAs were higher than in the normal pituitary tissue (P<0.05),and the expression in GH secreting-adenomas was the highest which has markedly differentia.The expression level of NFAs has no different compared with normal pituitary (P>0.05).Conclusion We supposed that PPARγ is an important molecular target in pituitary adenoma cells,especially for GH secreting-adenomas. Key words: PPARγ; Pituitary adenoma; GH secreting-adenoma; Nonfunctioning pituitary adenoma
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.