Correlation of insulin pretreatment and insulin binding of Amoeba proteus—a new technique for evaluation

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1. 1. Insulin binding sites of Amoeba proteus were analysed. The developed technique helped to compare equivalent pseudopodia membranes, according to their fluorescein-isothiocyanate (FITC)-insulin binding capacity. 2. 2. The number of insulin binding sites decrease in the control conditions as a fraction of time. 3. 3. The down regulation of binding sites (or similar phenomenon) lasts ~48 hr. 4. 4. Only low concentrations of insulin can induce a binding site elevation in the long term. 5. 5. Hormonal imprinting could be presented in Amoeba. 6. 6. The developed technique is suitable for detection of membrane dynamics in Amoeba proteus.