Correlation of histopathological observations of appendicitis tissue with glutathione, lipid peroxidation, and lactate dehydrogenase: An investigational study

Abstract

Objective: The present study was conducted to investigate the levels of antioxidant glutathione (GSH), lactate dehydrogenase (LDH), and lipid peroxidation (LPx) in the excised appendicitis tissue and investigate as to whether there is any correlation with the histopathological grading and clinical scoring. Materials and Methods: Appendix was collected from people requiring appendectomy ( n = 30) and people with normal appendix but requiring gastrointestinal (GI) surgery for some other GI diseases and willing for complementary appendectomy ( n = 4). The levels of GSH, LPx, and LDH were evaluated and statistically compared. In addition, statistical analysis is used to observe for correlation between these parameters with histological grading and Alvarado scores. Results: When compared to the normal appendix, in the tissues of appendicitis, the levels of LPx were high and that of LDH and GSH were significantly low. A negative correlation for GSH and positive correlation for LPx were observed. With respect to LDH, a negative correlation for tissue was observed with both histopathological and clinical grading. Multiple regression analysis for the biochemical endpoint in tissue showed that these parameters contribute 67.9 and 30.1% toward the histopathological and clinical grading, respectively. Conclusions: The results of the study indicate that the levels of LPx are increased and that of LDH and GSH are decreased in appendicitis and they are dependent on the pathological and clinical manifestation.