Correlation between TLR4 gene polymorphism and severe enterovirus 71 infection

Abstract

ObjectiveEnterovirus 71 (EV71) infection resulted in high mortality and disability in children. Immune response deficiency and cytokine genetic predispositions were associated with the severity of EV71 infection. We aim to evaluate the association between TLR4 gene polymorphisms and severity of EV71 infection in Chinese children. MethodsTLR4 gene polymorphisms were detected through improved multiplex ligation detection reaction technology. TLR4 expression was measured by Real-Time reverse transcriptase PCR and flow cytometry. The plasma levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were analyzed by enzyme-linked immunosorbent assay. ResultsThe frequency of rs10759932CC genotype and the C allele was significantly higher in cases with severe EV71 infection than those with mild infection. The levels of TLR4, serum TNF-α and IL-6 in cases with rs10759932CC genotype were also significantly elevated when compared to those with TT genotypes. ConclusionsThe rs10759932 polymorphism in TLR4 was associated with the severity of EV71 infection. The C allele of rs10759932 may be one of the risk factors of severe EV71 infection in Chinese children.