Abstract
It has been hypothesized that the cytosolic esterase-induced fluorescence intensity (CEIFI) from carboxy dimethyl fluorescein diacetate (CMFDA) in platelets may related to platelet functions. In the present study, we measured the change of CEIFI in platelets during storage, and examined the correlations of CEIFI with the in vitro functionality of stored platelets, including the ADP-induced aggregation activity, hypotonic shock response, expression of CD62P as well as platelet apoptosis. The CEIFI of fresh platelets, when tested at 10 μM CMFDA, the mean fluorescence intensity index (MFI) was 305.9 ± 49.9 (N = 80). After 1-day storage, it was 203.8 ± 34.4, the CEIFI of the stored platelets started to decline significantly, and reduced to 112.7 ±27.7 after 7-day storage. The change in CEIFI is highly correlated to all four functional parameters measured, with the correlation coefficients being 0.9813, 0.9848, -0.9945 and -0.9847 for the ADP-induced aggregation activity, hypotonic shock response (HSR), expression of CD62P and platelet apoptosis respectively. The above results show that the CEIFI measurement of platelets represents well the viability and functional state of in vitro stored platelets. This may be used as a convenient new method for quality evaluation for stored platelets if this result can be further validated by the following clinical trials.
Highlights
Platelet transfusion is the most effective treatment for hemorrhage patients with severely reduced platelet counts and with damaged or impaired platelet functions [1]
We have investigated the utility of cytosolic esterase-induced fluorescence intensity (CEIFI) of platelets stained by carboxy dimethyl fluorescein diacetate (CMFDA) as a physiological indicator for the functional state of stored platelets
The objective of the present study is to examine how well CEIFI of platelets is correlated to the functional aspects of stored platelets in vitro such as aggregation, activation, apoptosis and anti-hypotonic shock response
Summary
Platelet transfusion is the most effective treatment for hemorrhage patients with severely reduced platelet counts and with damaged or impaired platelet functions [1]. The quality of platelets directly affects the therapeutic effects of clinical transfusion. The Food and Drug Administration of the United States stipulates that platelets can be stored for five days at 22°C under horizontal vibration, but the method and threshold of functional evaluation of platelets. Correlation between the Functionality of Platelets and the CEIFI acquired singly during storage periods have not been specified. There are several assays for measuring certain aspects of platelet function, including the assessment of platelet adhesion, activation and aggregation, all of them have measuring certain aspects. The functional evaluation of platelet quality could not be made during storage because in vitro indicators that can accurately reflect the functional states and therapeutic efficacy of platelets have yet to be established and adopted. Any new assay that can better reflect the functional state and/or transfusion efficacy would be of great value
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