Abstract
The aim of this study is to assess the correlation between HBA and two other male diagnostic tests. Observational prospective study. The study includes 41 samples analysed at CIRH, Barcelona (Spain) following the protocol suggested by the manufacturer. Mean age of male patients was 35 years old. Sperm DNA fragmentation was assessed using the Halosperm kit (Halotech DNA SL, Madrid, Spain). To assess sperm morphology by high magnification we considered spermatozoa from groups III and IV, those spermatozoa with high vacuolization measured by inverted microscopy (Leica AM6000) at more than 6000x magnification. HBA test presents a higher negative correlation with high magnification (r=0.16) being statistically significant (p=0.012). On the contrary, the results of the HBA test are correlated positively with DNA fragmentation (r=0.05) although not statistically significant (p>0.05). The obtained results show that samples enriched with spermatozoa of bad morphology present low percentage of positive HBA. Furthermore, the integrity of the DNA does not seem to be related directly to the degree of sperm maturity. The lack of correlation could be due to the sample size or, maybe because the outsourcing of the hyaluronate receptor is made regardless of the degree of DNA integrity. It would be desirable to increase the number of samples to verify this hypothesis. It is necessary to deepen the relationship between the three sperm parameters to assess their predictive power.
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