Abstract
Full activity of hydrogenase is induced in autotrophic or heterotrophic Scenedesmus obliquus [strain D3 (Gaffron)] cells after 2 h under anaerobic adaptation. Semi‐aerobic conditions allow only an induction of 20% activity. Hydrogenase is completely inactivated by oxygen in cell free preparations. Semi‐aerobic adaptation of hydrogenase is enhanced by glucose and inhibited by sodium azide. It is concluded that respiration enhances hydrogenase activation by creating an oxygen free microclimate and by providing energy in the form of ATP.
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