Correlation between polymorphism of -152G/A and 7488T/C at IL-17 gene and levels of total serum IgE among 224 children with Asthma in Guiyang

Abstract

Objective To investigate the influences of interleukin 17A gene promoter -152G/A (IL-17A -152G/A) and interleukin 17F gene exon 7488 T/C (IL-17F 7488T/C) single nucleotide polymorphisms on children bronchial asthma and serum total IgE (TIgE) levels. Methods Genotype distribution of IL-17A -152G/A and IL-17F 7488T/C in 224 children with asthma and 150 healthy subjects was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Some samples were selected for PCR product sequencing and verification. IMMAGE800 specific protein analyzer from the Beckman Coulter test system was used to automatically detect serum TIgE levels in all subjects. Results Three genotypes of AA, AG and GG were detected at IL-17A -152G/A locus in both asthma and control groups. Differences in genotype frequency distribution were statistically significant between the two groups (P 0.05). Conclusion IL-17 gene polymorphism was correlated with the incidence of asthma in children in Guiyang area. IL-17A -152G/A polymorphism was an important candidate gene for asthma in Guiyang area and the variant allele A at -152G/A locus was correlated with increased serum TIgE levels. IL-17F 7488T/C polymorphism was also an important candidate gene for asthma in Guiyang. Children carrying the variant allele C at IL-17F 7488T/C locus showed a lower risk for asthma in Guiyang. IL-17F 7488T/C polymorphism had no influence on serum TIgE levels. Key words: Bronchial asthma; IL-17; -152G/A; 7488T/C; Single nucleotide polymorphism; Total serum IgE