Correlation between platelet membrane glycoproteinIIb HPA-3 polymorphism and the risk of ischemic stroke and outcomes in a Chinese Han population in Tianjin area

Abstract

Objective To investigate the correlation between platelet membrane glycoproteinⅡb HPA-3 polymorphism and the risk of ischemic stroke and outcomes in a Chinese Han population in Tianjin area. Methods Consecutive patients with acute ischemic stroke and sex- and age-matched healthy subjects were enrolled. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to detect glycoproteinⅡb HPA-3 polymorphism. Poor outcome was defined as the modified Rankin scale score >2 at 6 months. Results A total of 224 patients with acute ischemic stroke and 98 sex- and age-matched healthy subjects were enrolled. The bb genotype (45.09% vs. 15.06%; χ2=40.618, P<0.001) and b allele (63.84% vs. 33.67%; χ2=50.064, P<0.001) frequencies of the acute ischemic stroke group were significantly higher than those of the control group; multivariate logistic regression analysis showed that the bb genotype was an independent risk factor for ischemic stroke (odds ratio 5.838, 95% confidence interval 2.653-12.843; P<0.001). In each TOAST subtypes, the bb genotype (χ2=10.912, P=0.028) and b allele (χ2=11.801, P=0.019) frequencies of the large artery atherosclerosis group were significantly higher than those of other groups. The proportions of the patients with large artery atherosclerosis (64.52% vs. 29.63%; χ2=29.456, P<0.001) and bb genotype (66.13% vs. 37.04%; χ2=17.422, P<0.001), and b allele (75.00% vs. 59.57%; χ2=9.252, P=0.002) frequencies of the poor outcome group were significantly higher than those of the good outcome group; multivariate logistic regression analysis showed that the bb genotype was an independent risk factors for poor outcome in patients with ischemic stroke (odds ratio 3.842, 95% confidence interval 1.782-8.283; P=0.001). Conclusions The bb genotype may be an independent risk factor for the onset of ischemic stroke and poor outcomes in the Chinese Han population in Tianjin area. Key words: Stroke; Brain Ischemia; Platelet Glycoprotein GPIIb-IIIa Complex; Polymorphism, Genetic; Prognosis