Correlation between platelet membrane glycoprotein Hb HPA-3 polynmrphism and ischemic stroke in Chinese Han population

Abstract

Objective To investigate the distribution of gtycoprotein （GP） Ⅱ b HPA-3 polymorphism in Chinese Han population in Tianjin and its correlation with ischemic stroke. Methods The patients in this study were divided into either a ischerrfic stroke group （n = 150） or a control goup （n = 135）. Genotyping was conducted by using polymerase chain reactionrestriction fragrnent length polymorphism and was verified by sampling sequencing Each notype and allele frequency distribution and its correlation with ischemic stroke were compared. Results The ab notype, bb genotype and b allele frequency in the patient group were significantly higher than those in the control group （P =0. 000）, while aa otype and a allele frequency were significantly lower than those in the control group （P = 0. 000）. There were no significant differences in the frequencies of GP Ⅱ b genotype and b allele between the different gender and the age groups in the patient group. Although there were no significant differences in genotype frequencies between all etiologic subtypes, b allele frequency in the large artery atherosclerotic stroke suboup was siaificantly higher than that in the small vascular occlusive stroke subgroup and that in the cardioembolism subgroup （61.8% vs. 46. 7% vs. 47.5% ; X2 =6. 573, P =0. 037）. Multivariate logistic regression analysis showed that hypertension （odds ratio [OR] 7. 475, 95% confidence interval [CI]3. 700 - 15. 003; P = 0. 000） and b allele （OR 3. 678, 95% CI 1. 245 - 10. 863; P = 0. 018） were the independent risk factors for ischemic stroke. Conelusiom GP lI b HPA-3polymorphism may be associated with the risk of ischemic stroke onset. Carrying b allele may be an independent risk factor for ischemic stroke, especially large artery atherosclerotic stroke. Key words: Platelet Glycoprotein GP Ⅱ b- m a Complex; Polymorphism, Genetic; Stroke; BrainIschemia; Risk Factors