Abstract
Alfalfa (Medicago sativa L.) is from Fabaceae family that has several flavonoid compound in roots and shoots. in alfalfa the major phytoalexin is medicarpin. In this study, total phenolic and flavonoid compounds and phenylalanin ammonia lyase (PAL) activity in different stages of development were measured. In this research the concentration of medicarpin by HPLC were studied in different stages of development. The lowest and highest level of the concentration of medicapin were in seedling stage and budding stage of growth, respectively. The results indicated that contents of total phenolic compounds and total flavonoid and PAL activity increase with developmental stage ,but decrease in flowering stage of growth.
Highlights
Higer plants synthesized a wide variety of phenolic compounds during normal growth and development [8]
It is synthesized via the isoflavonoid branch of phenylpropanoid metabolism phenylalanin ammonia lyase ( PAL) is the enzyme which introduces phenylalanine to the phenylpropanoid pathway is involved in the first step committed to falvonoid biosynthesis [10, 12]
There was a correlation between PAL activity and total flavonoid concentration
Summary
Higer plants synthesized a wide variety of phenolic compounds during normal growth and development [8]. Medicago sativa L. from Fabaceae family is a perennial herbaceous plant. It has several phenolic and flavonoid compounds in root and shoot [3]. Medicarpin generally accumulate at relatively high concentration in the cell vacuole and are chemical associated with sugars. It is synthesized via the isoflavonoid branch of phenylpropanoid metabolism phenylalanin ammonia lyase ( PAL) is the enzyme which introduces phenylalanine to the phenylpropanoid pathway is involved in the first step committed to falvonoid biosynthesis [10, 12]. The aim was to determinate of the was a link PAL activiy and concentration medicarpin and development stages
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